目的 研究合柄铁线莲中黄酮苷类化学成分。方法 采用多种分离材料和方法,结合半制备液相分离合柄铁线莲中的黄酮苷类成分。运用波谱技术鉴定化合物的结构。结果 分离得到11个黄酮苷类化合物,分别鉴定为山柰酚-3-O-β-D-葡萄糖醛酸甲酯(1)、异牡荆素(2)、过山蕨素(山柰酚-3-O-β-D-葡萄糖基-7-α-L-鼠李糖苷)(3)、山柰酚-3-O-α-L-鼠李糖基-7-O-α-L-鼠李糖苷(4)、山柰酚-3-O-α-L-(4-O-乙酰基)鼠李糖基-7-O-α-L-鼠李糖苷(5)、山柰酚-3-O-(2-β-D-葡萄糖基)-α-L-鼠李糖基-7-O-α-L-鼠李糖苷(6)、染料木素-7-O-β-D-呋喃芹糖基-(1→6)-O-β-D-吡喃葡萄糖苷(7)、澳白檀苷(8)、槲皮素-3-O-β-D-(6″-n-丁基)葡萄糖醛酸酯(9)、槲皮素-3-O-β-D-葡萄糖醛酸甲酯(10)和异荭草素(11)。结论 化合物1～11均为首次从该植物中分离得到。
Abstract
OBJECTIVE To investigate the flavonoid glycosides in Clematis connata DC. METHODS Colum chromatography with different materials, such as RP-18, Sephadex LH-20 and silica gel, and semi preparative high performance liquid chromatography were used to isolate and purify the chemical constituents. Their structures were identified by spectroscopic analysis.RESULTS Eleven flavonoid glycosides were isolated and identified as kaempferol-3-O-β-D-glucopyranosyl methyl ester(1), isovitexin(2), kaempferol-3-O-β-D-glucopyranoside-7-α-L-rhamnopyranoside(3), kaempferol-3-O-α-L-rhamnopyranoside-7-α-L-rhamnopyranoside(4), kaempferol-3-O-α-L-(4-O-acetyl) rhamnopyranoside-7-α-L-rhamnopyranoside(5), kaempferol-3-O-(2-β-D-glucopyranosyl)-α-L-rhamnopyranoside-7-O-α-L-rhamnopyranoside(6), genistein-7-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside(7), lanceolarin(8), quercetin-3-O-β-D-(6″-n-butyl) glucuronide(9), quercetin-3-O-β-D-glucopyranosyl methyl ester(10), and isoorientin(11). CONCLUSION Compounds 1-11 are isolated from Clematis connata DC for the first time.
关键词
合柄铁线莲 /
黄酮苷 /
山柰酚-3-O-β-D-葡萄糖醛酸甲酯 /
山柰酚-3-O-β-D-葡萄糖基-7-α-L-鼠李糖苷
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Key words
Clematis connata DC /
flavonoid glycoside /
kaempferol-3-O-β-D-glucopyranosyl methyl ester /
kaempferol-3-O-β-D-glucopyranoside-7-α-L-rhamnopyranoside
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中图分类号：
R284
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参考文献
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脚注
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基金
国家自然科学基金资助项目(31260073);云南省应用基础研究计划资助项目(2013FZ103)
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